One vial of 2 x 106 cells, at P4 in Freezing Media. IMPORTANT: store the frozen cells in liquid nitrogen until you are ready to thaw and propagate them.
HeLa cells are the oldest and most commonly used human cancer cell lines in biomedical research. It is an immortal cell line derived from cervical carcer cells taken from a lady called Henrietta Lacks in 1951, which was found to be remarkably durable and prolific. HeLa-GFP stable cell line is transformed from the HeLa cell line and stably expresses the GFP fluorescent protein. Both GFP and geneticin-resistant genes are introduced into parental HeLa cells using retrovirus. Strong Green fluorescent signal can be visualized in every single cell under fluorescent microscope. The expression of GFP has been validated by western blot.
Images
Fluorescence microscope images.
Western blot analysis and Auto-western analysis.
Lane M: Protein marker. 1: HeLa/GFP 2: HeLa Rabbit anti-GFP: 130-11011 (1:1000)
Additional Materials Required
Dulbecco's Modified Eagles Medium (DMEM)
Fetal Bovine Serum (FBS)
Penicillin/Streptomycin
Trypsin
Phosphate-buffered saline (PBS)
DMSO
96-well white plate
Storage
Store the frozen cells in liquid nitrogen until you are ready to thaw and propagate them. It is strongly recommended that you propagate the cells following instructions as soon as possible upon arrival. An adequate number of frozen stocks must be made from early passages as cells will undergo genotypic changes. Genetic instability in transfected cells will result in a decreased responsiveness over time in normal cell culture conditions.
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