HeLa cells are the oldest and most commonly used human cancer cell lines in biomedical research. It is an immortal cell line derived from cervical carcer cells taken from a lady called Henrietta Lacks in 1951, which was found to be remarkably durable and prolific. HeLa- HLA-A:02:01 stable cell line is transformed from the HeLa cell line and stably expresses the HLA-A:02:01 protein. The expression of HLA-A:02:01 has been validated by western blot.
- Dulbecco's Modified Eagle Medium (DMEM)
- Fetal Bovine Serum (FBS)
- Penicillin/Streptomycin
- Trypsin
- Phosphate-buffered saline (PBS)
- DMSO
- 96-well white plate
Store the frozen cells in liquid nitrogen until you are ready to thaw and propagate them. It is strongly recommended that you propagate the cells following instructions as soon as possible upon arrival. An adequate number of frozen stocks must be made from early passages as cells will undergo genotypic changes. Genetic instability in transfected cells will result in a decreased responsiveness over time in normal cell culture conditions.
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