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规格

规格	8样本、2样本、2样本(产品加服务)、4样本、8样本(产品加服务)、4样本(产品加服务)
样本类型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
检测方法	Chemiluminescence
定量与否	Semi-Quantitative
载体	Membrane
适用种属	Human
检测靶标	alpha-Synuclein, ATG10, ATG12, ATG13, ATG3, ATG4A, ATG4B, ATG5, ATG7, Beclin, BNIP3L, DDR2, GABARAP, LC3a, LC3B, MSK1, NBS1, P62, RHEB, LAMP1 / CD107a
靶标数	20

详细描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ATG12 Gene Symbol: ATG12 APG12 APG12L Gene ID: 9140 Accession #: O94817	ATG7 Gene Symbol: ATG7 APG7L Gene ID: 10533 Accession #: O95352	ATG10 Gene Symbol: ATG10 APG10L PP12616 Gene ID: 83734 Accession #: Q9H0Y0	ATG13 Gene Symbol: ATG13 KIAA0652 Gene ID: 9776 Accession #: O75143	ATG3 Gene Symbol: ATG3 APG3 APG3L Gene ID: 64422 Accession #: Q9NT62
ATG4A Gene Symbol: ATG4A APG4A AUTL2 Gene ID: 115201 Accession #: Q8WYN0	ATG4B Gene Symbol: ATG4B APG4B AUTL1 KIAA0943 Gene ID: 23192 Accession #: Q9Y4P1	ATG5 Gene Symbol: ATG5 APG5L ASP Gene ID: 9474 Accession #: Q9H1Y0	Beclin Gene Symbol: BECN1 GT197 Gene ID: 8678 Accession #: Q14457	BNIP3L Gene Symbol: BNIP3L BNIP3A BNIP3H NIX Gene ID: 665 Accession #: O60238
DDR2 Gene Symbol: DDR2 NTRKR3 TKT TYRO10 Gene ID: 4921 Accession #: Q16832	GABARAP Gene Symbol: GABARAP FLC3B HT004 Gene ID: 11337 Accession #: O95166	LAMP1 (CD107a) Gene Symbol: LAMP1 Gene ID: 3916 Accession #: P11279 ELISA Kit: RayBio Human LAMP1/CD107a ELISA	LC3a Gene Symbol: MAP1LC3A Gene ID: 84557 Accession #: Q9H492	LC3B Gene Symbol: MAP1LC3B MAP1ALC3 Gene ID: 81631 Accession #: Q9GZQ8
NBS1 Gene Symbol: NBN NBS NBS1 P95 Gene ID: 4683 Accession #: O60934	P62 Gene Symbol: SQSTM1 ORCA OSIL Gene ID: 8878 Accession #: Q13501	RHEB Gene Symbol: RHEB RHEB2 Gene ID: 6009 Accession #: Q15382	MSK1 Gene Symbol: RPS6KA5 MSK1 Gene ID: 9252 Accession #: O75582	Alpha-synuclein Gene Symbol: SNCA NACP PARK1 Gene ID: 6622 Accession #: P37840

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Autophagy Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

相关产品

Anti-Gus

Anti-Xa21

Anti-HPT

Anti-CP4-EPSPS

文件

AAH-ATG-1.pdf

文献(3)

HS 3D-SeboSkin Model Enables the Preclinical Exploration of Therapeutic Candidates for Hidradenitis Suppurativa/Acne Inversa,Imanishi Ichiro, Asahina Ryota, Hayashi Shunji, Uchiyama Jumpei, Hisasue Masaharu, Yamasaki Masahiro, Murata Yoshiteru, Morikawa Shigeru, Mizutani Tetsuya, Sakaguchi Masahiro,BMC Veterinary Research ,(IF:2.792)
Species：	Human
Sample Type：	skin

View publications

Spermidine-Eugenol Supplement Preserved Inflammation-Challenged Intestinal Cells by Stimulating Autophagy,Maria António, Tânia Lima, Rita Ferreira, Margarida Fardilha, José Mesquita Bastos, Rui Vitorino, Ana L. Daniel-da-Silva,Analytical Methods ,(IF:3.1)
Species：	Human
Sample Type：	unknown

View publications

Unravelling inclusion body myositis using a patient-derived fibroblast model,Gonzalez Caleb, Williamson Sarah, Gammon Seth T., Glazer Sarah, Rhee Joon Haeng, Piwnica-Worms David,Communications Biology ,(IF:5.9)
Species：	Human
Sample Type：	unknown

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