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规格

规格	2样本、8样本、8样本(产品加服务)、4样本(产品加服务)、2样本(产品加服务)、4样本
样本类型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
检测方法	Chemiluminescence
定量与否	Semi-Quantitative
载体	Membrane
适用种属	Human
检测靶标	BCAM, CEACAM-1, E-Cadherin, E-Selectin, Nr-CAM, P-Cadherin, P-Selectin, NCAM-1 / CD56, VE-Cadherin / CDH5, PECAM-1 / CD31, ICAM-2 / CD102, ALCAM / CD166, TROP1 / EpCAM, ICAM-1 / CD54, ICAM-3 / CD50, L-Selectin / CD62L, VCAM-1 / CD106
靶标数	17

详细描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ALCAM (CD166) Gene Symbol: ALCAM Gene ID: 214 Accession #: Q13740 ELISA Kit: RayBio Human ALCAM ELISA	BCAM Gene Symbol: BCAM Gene ID: 4059 Accession #: P50895 ELISA Kit: RayBio Human BCAM ELISA	CEACAM-1 Gene Symbol: CEACAM1 Gene ID: 634 Accession #: P13688 ELISA Kit: RayBio Human CEACAM-1 ELISA	E-Cadherin Gene Symbol: CDH1 Gene ID: 999 Accession #: P12830 ELISA Kit: RayBio Human E-Cadherin ELISA	TROP1 (EpCAM) Gene Symbol: EPCAM Gene ID: 4072 Accession #: P16422 ELISA Kit: RayBio Human TROP1 ELISA
E-Selectin Gene Symbol: SELE Gene ID: 6401 Accession #: P16581 ELISA Kit: RayBio Human E-Selectin ELISA	ICAM-1 (CD54) Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362 ELISA Kit: RayBio Human sICAM-1 ELISA	ICAM-2 (CD102) Gene Symbol: ICAM2 Gene ID: 3384 Accession #: P13598 ELISA Kit: RayBio Human ICAM-2 ELISA	ICAM-3 (CD50) Gene Symbol: ICAM3 Gene ID: 3385 Accession #: P32942 ELISA Kit: RayBio Human ICAM-3 ELISA	L-Selectin (CD62L) Gene Symbol: SELL Gene ID: 6402 Accession #: P14151 ELISA Kit: RayBio Human L-Selectin ELISA
NCAM-1 (CD56) Gene Symbol: NCAM1 Gene ID: 4684 Accession #: P13591 ELISA Kit: RayBio Human NCAM-1 ELISA	NrCAM Gene Symbol: NrCAM Gene ID: 4897 Accession #: Q92823 ELISA Kit: RayBio Human NRCAM ELISA	P-Cadherin Gene Symbol: CDH3 Gene ID: 1001 Accession #: P22223 ELISA Kit: RayBio Human P-Cadherin ELISA	P-Selectin Gene Symbol: SELP Gene ID: 6403 Accession #: P16109 ELISA Kit: RayBio Human P-Selectin ELISA	PECAM-1 (CD31) Gene Symbol: PECAM1 Gene ID: 5175 Accession #: P16284 ELISA Kit: RayBio Human PECAM-1 ELISA
VCAM-1 (CD106) Gene Symbol: VCAM1 Gene ID: 7412 Accession #: P19320 ELISA Kit: RayBio Human VCAM-1 ELISA	VE-Cadherin (CDH5) Gene Symbol: CDH5 Gene ID: 1003 Accession #: P33151

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Adhesion Molecule Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.