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规格

规格	100 tests
物种	Hamster
定量/半定量	定量
特异性	Assay performance was unaffected by the presence of 10 ng of contaminating HEK293 gDNA.
兼用样本	DNA
检测方法	PCR
研究领域	Bioprocesses
灵敏度	1.331 pg/mL
货期	3-4周
运输方式	干冰
储存	-20°C

详细描述

Introduction

RayBio^® CHO Residual DNA Quantification Kit is a ready to use real-time PCR assay for the rapid and reliable detection of CHO genomic DNA in biomanufacturing products. This assay is highly sensitive (≤ 5 pg/mL), resistant to DNA fragmentation, and specific to CHO, with no cross-reactivity to unrelated DNA products. An internal positive control, RNAse P, is also detected in each reaction to monitor impact of potential PCR inhibitors.

Kit Components

Component	Size / Qty
PCR Master Mix	1 tube
Primer and Probe Mix	1 tube
CHO DNA Control	1 tube
Dilution Buffer	2 tubes

Additional Materials Required

Fluorescence PCR instrument capable of reading FAM (494 nm maximum absorption, 518 nm maximum emission) and ROX (580 nm maximum absorption, 623 nm maximum excitation)
Compatible PCR Plate and optical film
Vortex Mixer
Microcentrifuge
Pipettes
Sterile nuclease-free pipette tips (barrier tips recommended) and microfuge tubes

Contact our technical support team for questions about compatibility: techsupport@raybiotech.com

Assay Procedure Summary

Reagent preparation
Sample Loading
PCR Amplification
Results and Analysis

Storage/Stability

The kit can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C (not exceeding 12 months since date of manufacture). If using kit over multiple tests, 10x Primers and Probe Mix and CHO DNA Control should be sub-aliquoted and re-frozen before first use to prevent run-to-run variability from repetitive freeze thaws.