GCGC黄金城【中国区】官方网站

规格

规格	48 tests, 96 tests, 192 tests, 480 tests
物种	人
蛋白ID	P10636
基因ID	4137
蛋白名称	Phosphorylated Tau at Threonine-217 (p-Tau217); Phosphorylated Tau at Threonine-181 (p-Tau181)
定量/半定量	定量
靶标	2
特异性	No detectable or significant cross reactivity was detected between antibodies or targets.
适用样本	Cell Culture Supernatants, Plasma, Serum
载体	Fluorescent Microbeads
检测方法	Flow Cytometry
设计原则	Sandwich-based
研究领域	Alzheimer's Disease
检测范围	68.6 pg/mL - 50,000 pg/mL
货期	2-3周
运输	干冰
储存	4°C

详细描述

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µL or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

pTau217 and pTau181

Kit Components

RayPlex® Multiplex Bead Cocktail
Assay Diluent
Wash Buffer
RayBio® Human Serum/Plasma Diluent
Lyophilized Protein Standard Mix
Biotinylated Detection Antibody Cocktail
PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate

Additional Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue (or green) and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
PBS
1.5 mL polypropylene microcentrifuge tubes or similar
Microcentrifuge
Recommended: Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Assay Procedure Summary

Prepare Reagents, Standards, and Samples
Add RayPlex® Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.